Peptidová kalkulačka - Veyrona Labs

VEYRONA LABS

Peptide calculator

Enter the peptide content, water volume and the amount you want to measure. The calculator shows the volume in millilitres and on the syringe scale.

1 Peptide content in the vial

2 How much water are you adding?

Enter the volume of bacteriostatic water or another suitable solvent in millilitres (ml).

3 How much peptide do you want to measure?

µg means micrograms (also written as mcg). mg means milligrams. 1 mg = 1,000 µg; for example, 0.5 mg = 500 µg.

4 U‑100 syringe capacityOn a U‑100 syringe, 100 scale units equal 1 ml. One scale unit is therefore 0.01 ml.

CALCULATION RESULT

Enter all three values to see the result automatically.

The calculator and information below are for preparing research solutions. Products are for research use only, not for use in humans.

CLEAR AND SIMPLE

How to reconstitute peptides

Reconstitution means dissolving a lyophilised, or freeze-dried, peptide in a suitable solvent. The powder in the vial becomes a solution for further laboratory work. Choose the solvent and volume according to the specific peptide and research protocol.

Prepare your workspace

Work on a clean surface. Prepare the vial, a suitable solvent, sterile measuring and transfer equipment, gloves and supplies required by your laboratory protocol. Check the product label and packaging integrity before starting.

Choose a suitable solvent

Peptides differ in solubility. Use bacteriostatic water only if it is suitable for the product and experiment. Some peptides require a different solution or buffer. Follow the product instructions and research protocol.

Prepare the vial

Let a vial removed from cold storage reach room temperature while still closed before opening, to limit moisture condensation. Handle the closure and equipment according to your laboratory’s aseptic procedure.

Add the measured solvent

Measure the chosen volume and add the liquid slowly along the inside wall of the vial. Mix gently by moving the vial; avoid vigorous shaking and foaming unless the protocol specifies otherwise.

Check dissolution and label the solution

Check that the peptide has dissolved as expected. If particles, cloudiness or gel remain, review the dissolution conditions. Label the vial with the name, batch, preparation date, solvent and concentration.

Follow the storage conditions

Protect the peptide from moisture and direct light. Determine the storage temperature and duration from the specific product data; one storage period does not apply to all peptides. If freezing is permitted by the protocol, divide the solution into smaller portions to limit repeated thawing.

What do mg, µg and scale units mean?

mg (milligrams) and µg (micrograms, also mcg) express the amount of peptide. ml (millilitres) express the volume of liquid. U‑100 scale units are volume markings on the syringe, not an amount of peptide.

1 mg = 1 000 µg0,5 mg = 500 µg10 U‑100 scale units = 0,1 ml

The same number of scale units can contain different amounts of peptide depending on the solution concentration. Adding more water to the same vial content gives a lower concentration.

Technical references: Thermo Fisher Scientific — peptide preparation and storage · Merck / Sigma-Aldrich — peptide dissolution